A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.
نویسندگان
چکیده
We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.
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عنوان ژورنال:
- Chemical communications
دوره 51 57 شماره
صفحات -
تاریخ انتشار 2015